產品名稱：Cortisol RIA
產    地：Demeditec 
產品貨號：DE28100
產品規格：100 Tests
產品說明：
Special remarks:
Introduction
Cortisol is the main glucocorticoid which is produced by the adrenal cortex. It regulates carbohydrate, protein, fat and purine metabolism, electrolyte and water balance. Cortisol has a role in blood pressure regulation and resolution of inflammation. The determination of cortisol levels can be used for diagnosing the functional disturbances of the hypothalamus-pituitary-adrenal cortex (HPA) axis. In normal individuals cortisol secretion has a characteristic rhythm: the cortisol level is the highest in the morning, by the evening it decreases to approximately half that level.
The above test kit measures the total (unconjugated) Cortisol in serum. There is also a method available for the measurement of free Cortisol in saliva. The metabolic activity of any steroid is just represented by the free hormone fraction which represents less than 5% of the total. The non-invasive sampling of saliva samples offers another advantage of salivary testing. Demeditec is offering such a testkit in ELISA format with the catalogue number DES6611.
Principle of the method
This assay is based on the competition between unlabelled cortisol and fixed quantity of 125I-labelled cortisol for limited number of binding sites on cortisol specific antibody. Allowing to react a fixed amount of tracer and antibody with different amounts of unlabelled ligand, the amount of tracer bound by the antibody will be inversely proportional to the concentration of unlabelled ligand. During a 2-hour incubation period with continuous agitation, immuno-complex is immobilized on the reactive surface of test tubes. After incubation the reaction mixture is discarded, and the radioactivity is measured in a gamma counter. The concentration of antigen is inversely proportional to the radioactivity measured in test tubes. By plotting binding values against a series of calibrators containing known amounts of cortisol, a calibration curve is constructed, from which the unknown concentration of cortisol in patient samples can determined.
上海玉博生物技術有限公司在為生命科學領域提供豐富的產品與信息資源方面處于****地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫學領域的應用。

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